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由于家兔性情溫順且易飼養(yǎng),綿陽常用于藥物對(duì)心臟、血壓、呼吸的影響及有機(jī)磷農(nóng)藥中毒和解救的實(shí)驗(yàn),也可用于研究藥物對(duì)中樞神經(jīng)的影響、體溫實(shí)驗(yàn)、熱原檢查及避孕藥的研究。新余南昌黑豚種苗價(jià)格九江上饒撫州宜春吉安黑豚鼠養(yǎng)殖基地貓、與家兔比較,貓對(duì)外科手術(shù)的耐受性強(qiáng),血壓穩(wěn)定,故常用于血壓實(shí)驗(yàn),也常用于心血管藥物及中樞神經(jīng)系統(tǒng)藥物的研究。
捉拿法有兩種、一種是用右手提起小鼠尾部,內(nèi)江實(shí)驗(yàn)鼠將其放在鼠籠蓋上或其他粗糙面上,向后上方輕拉,此時(shí)小鼠前肢緊緊抓住粗糙面,福建省福州漳州黑豚種苗基地價(jià)格 泉州寧德白豚鼠養(yǎng)殖場(chǎng)迅速用左手拇指和食指捏住小鼠頸背部皮膚并用小指和手掌夾持其尾根部固定于手中。另一種方法是只用左手抓住小鼠尾部,然后用拇指及食指捏住其頸部皮膚,然后再用手掌尺側(cè)及小指夾住其尾根。為先種方法簡(jiǎn)單易學(xué),第二種方法較難,但捉拿快速,給藥速度快。
斷頭取血 這是較常用且簡(jiǎn)便的一種取血方法,操作時(shí)一手抓住動(dòng)物,另一只手用剪刀剪掉其頭部,立即將鼠頸部向下,提起動(dòng)物,并對(duì)準(zhǔn)已備好的容器(容器內(nèi)事先放入抗凝劑),使鼠血快速滴入容器內(nèi)??魟?dòng)脈或眶靜脈取血 將動(dòng)物倒持,然后壓迫眼球,使其突出充血后,用止血鉗迅速摘除眼球,眼眶內(nèi)會(huì)很快流出血液,讓血滴入加有抗凝劑的玻璃器皿內(nèi),直至血流不出為止。一般情況下可取得相當(dāng)于動(dòng)物體重 4%~5% 的血液量。血流完動(dòng)物即死亡,此法只適用于一次性取血。
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Due to the gentle temperament and easy feeding of rabbits, Mianyang is commonly used for experiments on the effects of drugs on the heart, blood pressure, and respiration, as well as for experiments on organophosphorus pesticide poisoning and rescue. It can also be used for studying the effects of drugs on the central nervous system, body temperature experiments, pyrogen tests, and research on contraceptives. Compared with rabbits, cats have strong tolerance to surgical procedures and stable blood pressure, which is why they are often used in blood pressure experiments, as well as in the study of cardiovascular and central nervous system drugs.
There are two ways to capture the mouse. One is to lift the tail of the mouse with the right hand. The Neijiang experimental mouse places it on the cage cover or other rough surface, and gently pulls it back and up. At this time, the mouse's forelimbs tightly grasp the rough surface, quickly pinch the skin on the back of the mouse's neck with the left thumb and index finger, and clamp the root of the tail with the little finger and palm to fix it in the hand. Another method is to grab the tail of the mouse with only the left hand, then pinch the neck skin with the thumb and index finger, and then clamp the root of the tail with the ulnar side of the palm and little finger. The first method is simple and easy to learn, while the second method is more difficult, but it is fast to capture and administer drugs.
Cutting off the head to take blood is a commonly used and simple method of taking blood. When operating, one hand grabs the animal, while the other hand uses scissors to cut off its head. Immediately, the mouse's neck is lowered, the animal is lifted, and the prepared container (pre placed with anticoagulant) is aligned to allow the mouse blood to quickly drip into the container. Take blood from the orbital artery or vein, hold the animal upside down, and then compress the eyeball to protrude and congestion. After that, use a hemostatic forceps to quickly remove the eyeball. Blood will quickly flow out of the orbit, allowing it to drip into a glass container filled with anticoagulants until no blood can flow out. Generally, a blood volume equivalent to 4% to 5% of the animal's body weight can be obtained. Animals die after completing blood flow, and this method is only applicable to one-time blood collection.